1. A液(0.2M磷酸二氢钠水溶液): NaH2 PO4 ・H2 O 27.6g,溶于蒸馏水中,稀释至1000ml。

2. B液(0.2M磷酸氢二钠水溶液):Na2 HPO4 ・7H2 O 53.6g (或Na2 HPO4 ・12 H2 O71.6g或Na2 HPO4 ・2 H2 O 35.6g ) 加蒸馏水溶解,加水至1000ml。

3. 不同pH值磷酸盐缓冲液(PB)缓冲液的配制

A液X ml (参照下表) 中,加入B液Y ml,为0.2M PB。若再加蒸馏水至200ml则成0.1M PB。

PHX mlY mlpHX mlY ml5.793.56.56.945.055.05.892.08.07.039.061.05.990.010.07.133.067.06.087.712.37.228.072.06.185.015.07.323.077.06.281.518.57.419.081.06.377.522.57.516.084.06.473.526.57.613.087.06.568.531.57.710.090.06.662.537.57.88.591.56.756.543.57.97.093.06.851.049.08.05.394.7

若先分别配成0.1M,1/15M 和1/200 M的A液和B液,可按下表配成相应浓度所需pH值的PB

KH2 PO4 或NaH2 PO4 H2 O (ml )Na2 HPO4 (ml )pH ( M/10 )pH( M/15 )pH( M/200 )4645.675.745.944555.785.836.064465.865.916.144375.945.996.224286.026.076.304196.086.146.3640106.126.196.4239116.176.246.4738126.236.286.5737136.286.326.6136146.336.376.6535156.396.416.6834166.416.456.7233176.456.496.7532186.496.536.7931196.536.566.8230206.556.596.8829216.586.636.9128226.616.686.9527236.656.727.0026246.706.767.0525256.766.817.0924266.816.867.1323276.846.917.1622286.896.947.1821296.896.967.2020306.916.987.2219316.947.017.2518326.977.037.2617337.007.057.3116347.027.077.3415357.067.117.3814367.107.157.4113377.147.207.4512387.177.247.4511397.247.287.5110407.307.337.599417.367.407.658427.427.497.707437.497.547.756447.577.617.805457.657.697.873477.817.858.052487.927.978.15

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